ASK1

All methods over the ±5 window, side by side.

PSPA

CDDM

MLP-attr

Normalized values from positional scanning peptide array

PSPA measures pT, so at flanking positions the pS row is a duplicate of the pT row. At position 0 the acceptor identity (pS, pT, pY) is genuine.

Position-wise Probabilities

Log-Odds: Probabilities / STY Background

Position-wise Probabilities (±5 window)

Log-Odds (±5 window)

Motifs stratified by phospho-acceptor (S / T / Y)

K-means sub-motif clusters

Signed attribution PSSM distilled from the CDDM discriminative MLP (in-silico saturation mutagenesis). Letters above 0 are enriched, below are depleted; this is not a probability distribution.

MLP-attribution PSSM (±5, signed)

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substrate_uniprot	site	source	substrate_genes	site_seq
A5A3E0	T949	Sugiyama	POTEF A26C1B	TVAsSSSLEKsyELPDGQVItIGNERFRCPEALFQPCFLGM
A5A3E0	Y940	Sugiyama	POTEF A26C1B	ALDFEQEMATVAsSSSLEKsyELPDGQVItIGNERFRCPEA
O00151	T80	Sugiyama	PDLIM1 CLIM1 CLP36	NMTHLEAQNRIKGCTDNLtLtVARsEHKVWsPLVtEEGKRH
O15530	S394	EPSD\|PSP	PDPK1 PDK1	GNyDNLLSQFGCMQVSSSSssHsLsASDTGLPQRSGsNIEQ
O15530	S398	EPSD\|PSP	PDPK1 PDK1	NLLSQFGCMQVSSSSssHsLsASDTGLPQRSGsNIEQYIHD
O43423	T101	Sugiyama	ANP32CP PP32R1	EKCPNLTHLYLSGNKIKDLstIEPLKQLENLKSLDLFNCEV
O60361	Y136	Sugiyama	NME2P1	SLRFKPEELVDYKSCAHDWVyE___________________
O95626	T105	Sugiyama	ANP32D PP32R2	EKCPNLIHLNLSGNKIKDLstIEPLKKLENLESLDLFTCEV
P02786	T143	Sugiyama	TFRC	yWDDLKRKLsEKLDstDFTGtIKLLNENsyVPREAGsQKDE
P04075	S39	Sugiyama	ALDOA ALDA	IAHRIVAPGKGILAADEstGsIAKRLQsIGtENtEENRRFy
P06733	Y44	Sugiyama	ENO1 ENO1L1 MBPB1 MPB1	LFtsKGLFRAAVPsGAstGIyEALELRDNDKtRYMGKGVSK
P07195	T323	Sugiyama	LDHB	sVINQKLKDDEVAQLKKsADtLWDIQKDLKDL_________
P07858	T95	Sugiyama	CTSB CPSB	TEDLKLPAsFDAREQWPQCPtIKEIRDQGSCGSCWAFGAVE
P07900	S53	Sugiyama	HSP90AA1 HSP90A HSPC1 HSPCA	IINtFySNKEIFLRELIsNssDALDKIRyEsLtDPsKLDsG
P08238	Y301	Sugiyama	HSP90AB1 HSP90B HSPC2 HSPC3 HSPCB	ELNKtKPIWtRNPDDItQEEyGEFyKsLtNDWEDHLAVKHF
P08238	Y305	Sugiyama	HSP90AB1 HSP90B HSPC2 HSPC3 HSPCB	tKPIWtRNPDDItQEEyGEFyKsLtNDWEDHLAVKHFSVEG
P10809	T115	Sugiyama	HSPD1 HSP60	AKLVQDVANNtNEEAGDGtttAtVLARSIAKEGFEKISKGA
P10809	T117	Sugiyama	HSPD1 HSP60	LVQDVANNtNEEAGDGtttAtVLARSIAKEGFEKISKGANP
P11142	S40	Sugiyama	HSPA8 HSC70 HSP73 HSPA10	VFQHGKVEIIANDQGNRttPsyVAFtDtERLIGDAAKNQVA
P11142	T163	Sugiyama	HSPA8 HSC70 HSP73 HSPA10	VVtVPAyFNDsQRQAtKDAGtIAGLNVLRIINEPtAAAIAy
P11142	T226	Sugiyama	HSPA8 HSC70 HSP73 HSPA10	DVSILTIEDGIFEVKstAGDtHLGGEDFDNRMVNHFIAEFK
P11142	T64	Sugiyama	HSPA8 HSC70 HSP73 HSPA10	FtDtERLIGDAAKNQVAMNPtNtVFDAKRLIGRRFDDAVVQ
P11142	T66	Sugiyama	HSPA8 HSC70 HSP73 HSPA10	DtERLIGDAAKNQVAMNPtNtVFDAKRLIGRRFDDAVVQsD
P11142	Y41	Sugiyama	HSPA8 HSC70 HSP73 HSPA10	FQHGKVEIIANDQGNRttPsyVAFtDtERLIGDAAKNQVAM
P13489	S178	Sugiyama	RNH1 PRI RNH	SCEPLASVLRAKPDFKELtVsNNDINEAGVRVLCQGLKDSP
P13639	T5	Sugiyama	EEF2 EF2	________________MVNFtVDQIRAIMDKKANIRNMsVI
P13667	T449	Sugiyama	PDIA4 ERP70 ERP72	AATQFWRSKVLEVAKDFPEytFAIADEEDyAGEVKDLGLsE
P13667	Y458	Sugiyama	PDIA4 ERP70 ERP72	VLEVAKDFPEytFAIADEEDyAGEVKDLGLsEsGEDVNAAI
P14618	T50	Sugiyama	PKM OIP3 PK2 PK3 PKM2	MCRLDIDsPPItARNtGIICtIGPAsRsVEtLKEMIKsGMN
P17066	S42	Sugiyama	HSPA6 HSP70B'	VFQQGRVEILANDQGNRTtPsyVAFtDtERLVGDAAKsQAA
P17066	Y43	Sugiyama	HSPA6 HSP70B'	FQQGRVEILANDQGNRTtPsyVAFtDtERLVGDAAKsQAAL
P19338	S460	Sugiyama	NCL	ADAEKTFEEKQGtEIDGRsIsLyytGEKGQNQDYRGGKNST
P22392	Y151	Sugiyama	NME2 NM23B	SLWFKPEELVDyKSCAHDWVyE___________________
P23588	S207	Sugiyama	EIF4B	DRNRDsDKtDTDWRARPAtDsFDDyPPRRGDDsFGDKYRDR
P30086	Y169	Sugiyama	PEBP1 PBP PEBP	FKVAsFRKKyELRAPVAGtCyQAEWDDyVPKLyEQLsGK__
P34931	S42	Sugiyama	HSPA1L	VFQHGKVEIIANDQGNRttPsyVAFtDtERLIGDAAKNQVA
P34931	Y43	Sugiyama	HSPA1L	FQHGKVEIIANDQGNRttPsyVAFtDtERLIGDAAKNQVAM
P38646	T116	Sugiyama	HSPA9 GRP75 HSPA9B mt-HSP70	DGERLVGMPAKRQAVtNPNNtFyAtKRLIGRRYDDPEVQKD
P38936	S98	GPS6\|SIGNOR\|ELM\|iPTMNet\|EPSD\|PSP	CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1	LPtGPRRGRDELGGGRRPGtsPALLQGTAEEDHVDLsLSCT
P39687	T105	Sugiyama	ANP32A C15orf1 LANP MAPM PHAP1	EKCPNLTHLNLsGNKIKDLstIEPLKKLENLKsLDLFNCEV
P45983	T183	EPSD\|PSP	MAPK8 JNK1 PRKM8 SAPK1 SAPK1C	CTLKILDFGLARTAGtsFMMtPyVVtRyYRAPEVILGMGYK
P45983	Y185	EPSD\|PSP	MAPK8 JNK1 PRKM8 SAPK1 SAPK1C	LKILDFGLARTAGtsFMMtPyVVtRyYRAPEVILGMGYKEN
P45985	T261	GPS6\|SIGNOR\|ELM\|iPTMNet\|EPSD\|PSP	MAP2K4 JNKK1 MEK4 MKK4 PRKMK4 SEK1 SERK1 SKK1	GNIKLCDFGIsGQLVDsIAKtRDAGCRPyMAPERIDPSASR
P46734	S218	SIGNOR\|EPSD\|PSP	MAP2K3 MEK3 MKK3 PRKMK3 SKK2	INKEGHVKMCDFGISGyLVDsVAKtMDAGCKPYMAPERINP
P48741	S42	Sugiyama	HSPA7 HSP70B	VFQQGRVEILANDQGNRTtPsyVAFtDtERLVGDAAKsQAA
P48741	Y43	Sugiyama	HSPA7 HSP70B	FQQGRVEILANDQGNRTtPsyVAFtDtERLVGDAAKsQAAL
P52564	S207	EPSD\|PSP	MAP2K6 MEK6 MKK6 PRKMK6 SKK3	INALGQVKMCDFGISGyLVDsVAKtIDAGCKPyMAPERINP
P54652	S41	Sugiyama	HSPA2	VFQHGKVEIIANDQGNRttPsyVAFtDtERLIGDAAKNQVA
P54652	T229	Sugiyama	HSPA2	DVSILTIEDGIFEVKstAGDtHLGGEDFDNRMVSHLAEEFK
P54652	Y42	Sugiyama	HSPA2	FQHGKVEIIANDQGNRttPsyVAFtDtERLIGDAAKNQVAM
P60174	T217	Sugiyama	TPI1 TPI	VsDAVAQstRIIyGGsVtGAtCKELASQPDVDGFLVGGAsL
P60660	T103	Sugiyama	MYL6	QGtyEDyVEGLRVFDKEGNGtVMGAEIRHVLVtLGEKMTEE
P60709	S323	Sugiyama	ACTB	ttMyPGIADRMQKEItALAPstMKIKIIAPPERKYsVWIGG
P60709	T249	Sugiyama	ACTB	tAAssssLEKsyELPDGQVItIGNERFRCPEALFQPsFLGM
P60709	T324	Sugiyama	ACTB	tMyPGIADRMQKEItALAPstMKIKIIAPPERKYsVWIGGs
P60709	Y240	Sugiyama	ACTB	ALDFEQEMAtAAssssLEKsyELPDGQVItIGNERFRCPEA
P62736	S325	Sugiyama	ACTA2 ACTSA ACTVS GIG46	tTMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGG
P62736	T251	Sugiyama	ACTA2 ACTSA ACTVS GIG46	tAAssssLEKsyELPDGQVItIGNERFRCPETLFQPSFIGM
P62736	T326	Sugiyama	ACTA2 ACTSA ACTVS GIG46	TMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGGS
P62736	Y242	Sugiyama	ACTA2 ACTSA ACTVS GIG46	ALDFENEMAtAAssssLEKsyELPDGQVItIGNERFRCPET
P62942	T7	Sugiyama	FKBP1A FKBP1 FKBP12	______________MGVQVEtIsPGDGRtFPKRGQtCVVHy
P63261	S323	Sugiyama	ACTG1 ACTG	ttMyPGIADRMQKEItALAPstMKIKIIAPPERKYsVWIGG
P63261	T249	Sugiyama	ACTG1 ACTG	tAAssssLEKsyELPDGQVItIGNERFRCPEALFQPsFLGM
P63261	T324	Sugiyama	ACTG1 ACTG	tMyPGIADRMQKEItALAPstMKIKIIAPPERKYsVWIGGs
P63261	Y240	Sugiyama	ACTG1 ACTG	ALDFEQEMAtAAssssLEKsyELPDGQVItIGNERFRCPEA
P63267	S324	Sugiyama	ACTG2 ACTA3 ACTL3 ACTSG	tTMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGG
P63267	T250	Sugiyama	ACTG2 ACTA3 ACTL3 ACTSG	tAAssssLEKsyELPDGQVItIGNERFRCPETLFQPSFIGM
P63267	T325	Sugiyama	ACTG2 ACTA3 ACTL3 ACTSG	TMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGGS
P63267	Y241	Sugiyama	ACTG2 ACTA3 ACTL3 ACTSG	ALDFENEMAtAAssssLEKsyELPDGQVItIGNERFRCPET
P68032	S325	Sugiyama	ACTC1 ACTC	tTMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGG
P68032	T251	Sugiyama	ACTC1 ACTC	tAAssssLEKsyELPDGQVItIGNERFRCPETLFQPSFIGM
P68032	T326	Sugiyama	ACTC1 ACTC	TMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGGS
P68032	Y242	Sugiyama	ACTC1 ACTC	ALDFENEMAtAAssssLEKsyELPDGQVItIGNERFRCPET
P68133	S325	Sugiyama	ACTA1 ACTA	TTMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGG
P68133	T251	Sugiyama	ACTA1 ACTA	tAAssssLEKsyELPDGQVItIGNERFRCPETLFQPSFIGM
P68133	T326	Sugiyama	ACTA1 ACTA	TMYPGIADRMQKEItALAPstMKIKIIAPPERKYSVWIGGS
P68133	Y242	Sugiyama	ACTA1 ACTA	ALDFENEMAtAAssssLEKsyELPDGQVItIGNERFRCPET
P68363	S439	Sugiyama	TUBA1B	SEAREDMAALEKDyEEVGVDsVEGEGEEEGEEy________
P68363	Y432	Sugiyama	TUBA1B	GMEEGEFSEAREDMAALEKDyEEVGVDsVEGEGEEEGEEy_
Q05682	T86	Sugiyama	CALD1 CAD CDM	VEVNAQNsVPDEEAKTTtTNtQVEGDDEAAFLERLARREER
Q13283	T241	Sugiyama	G3BP1 G3BP	ETAPEDAQKsssPAPADIAQtVQEDLRtFsWAsVtsKNLPP
Q14568	S53	Sugiyama	HSP90AA2P HSP90AA2 HSPC2 HSPCAL3	IINTFYSNKEIFLRELIsNssDALDKIWYESLTDPSKLDSG
Q15056	T100	Sugiyama	EIF4H KIAA0038 WBSCR1 WSCR1	KFKGFCyVEFDEVDsLKEALtyDGALLGDRsLRVDIAEGRK
Q15691	S40	EPSD\|PSP	MAPRE1	MLAWINESLQLNLTKIEQLCsGAAYCQFMDMLFPGSIALKK
Q15691	T154	EPSD\|PSP	MAPRE1	EtAVAPsLVAPALNKPKKPLtsssAAPQRPIstQRTAAAPK
Q15691	T206	EPSD\|PSP	MAPRE1	VGNGDDEAAELMQQVNVLKLtVEDLEKERDFyFGKLRNIEL
Q16539	T180	EPSD\|PSP	MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A	EDCELKILDFGLARHtDDEMtGyVAtRWYRAPEIMLNWMHY
Q16539	Y182	EPSD\|PSP	MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A	CELKILDFGLARHtDDEMtGyVAtRWYRAPEIMLNWMHYNQ
Q562R1	T250	Sugiyama	ACTBL2	RAAAssSPERsyELPDGQVItIGNERFRCPEAIFQPSFLGI
Q562R1	Y241	Sugiyama	ACTBL2	ALDFEQEMVRAAAssSPERsyELPDGQVItIGNERFRCPEA
Q6S8J3	T949	Sugiyama	POTEE A26C1A POTE2	tAAssssLEKsyELPDGQVItIGNERFRCPEALFQPCFLGM
Q6S8J3	Y940	Sugiyama	POTEE A26C1A POTE2	ALDFEQEMAtAAssssLEKsyELPDGQVItIGNERFRCPEA
Q6ZN16	T662	Sugiyama	MAP3K15 ASK3	TLEYEYDHDANGERVVLGKGtYGIVYAGRDLSNQVRIAIKE
Q6ZN16	Y690	Sugiyama	MAP3K15 ASK3	RDLSNQVRIAIKEIPERDSRySQPLHEEIALHKYLKHRNIV
Q71U36	S439	Sugiyama	TUBA1A TUBA3	SEAREDMAALEKDyEEVGVDsVEGEGEEEGEEy________
Q71U36	Y432	Sugiyama	TUBA1A TUBA3	GMEEGEFSEAREDMAALEKDyEEVGVDsVEGEGEEEGEEy_
Q8IUQ4	T235	PSP	SIAH1 HUMSIAH	RKQAENFAYRLELNGHRRRLtWEAtPRSIHEGIATAIMNSD
Q8IUQ4	T239	PSP	SIAH1 HUMSIAH	ENFAYRLELNGHRRRLtWEAtPRSIHEGIATAIMNSDCLVF
Q8IUQ4	T70	PSP	SIAH1 HUMSIAH	PPILQCQSGHLVCSNCRPKLtCCPtCRGPLGSIRNLAMEKV
Q8IUQ4	T74	PSP	SIAH1 HUMSIAH	QCQSGHLVCSNCRPKLtCCPtCRGPLGSIRNLAMEKVANSV
Q969S3	S314	SIGNOR\|EPSD\|PSP	ZNF622 ZPR9	YLGEKVGVGKICLWCNEKGKsFYStEAVQAHMNDKSHCKLF
Q969S3	T318	SIGNOR\|EPSD\|PSP	ZNF622 ZPR9	KVGVGKICLWCNEKGKsFYStEAVQAHMNDKSHCKLFTDGD
Q99683	S966	GPS6\|ELM\|iPTMNet\|EPSD\|PSP	MAP3K5 ASK1 MAPKKK5 MEKK5	KTQPKLSALSAGsNEYLRsIsLPVPVLVEDtssssEYGsVs
Q99683	T690	Sugiyama	MAP3K5 ASK1 MAPKKK5 MEKK5	LLEYDYEYDENGDRVVLGKGtYGIVYAGRDLSNQVRIAIKE
Q99683	T779	Sugiyama	MAP3K5 ASK1 MAPKKK5 MEKK5	GGSLSALLRSKWGPLKDNEQtIGFYTKQILEGLKYLHDNQI
Q99683	T813	GPS6\|SIGNOR\|EPSD\|PSP	MAP3K5 ASK1 MAPKKK5 MEKK5	YLHDNQIVHRDIKGDNVLINtYSGVLKISDFGtSKRLAGIN
Q99683	T838	GPS6\|SIGNOR\|EPSD\|PSP\|Sugiyama	MAP3K5 ASK1 MAPKKK5 MEKK5	LKISDFGtSKRLAGINPCTEtFTGtLQyMAPEIIDKGPRGY
Q99683	T842	GPS6\|SIGNOR\|EPSD\|PSP\|Sugiyama	MAP3K5 ASK1 MAPKKK5 MEKK5	DFGtSKRLAGINPCTEtFTGtLQyMAPEIIDKGPRGYGKAA
Q99683	Y718	Sugiyama	MAP3K5 ASK1 MAPKKK5 MEKK5	RDLSNQVRIAIKEIPERDSRySQPLHEEIALHKHLKHKNIV
Q99683	Y845	Sugiyama	MAP3K5 ASK1 MAPKKK5 MEKK5	tSKRLAGINPCTEtFTGtLQyMAPEIIDKGPRGYGKAADIW
Q9BQE3	Y432	Sugiyama	TUBA1C TUBA6	GMEEGEFSEAREDMAALEKDyEEVGADsADGEDEGEEy___
Q9BYX7	T249	Sugiyama	POTEKP ACTBL3 FKSG30	MAASSSSVEKsyELPDGQVItIGNERFRCPEALFQPCFLGM
Q9BYX7	Y240	Sugiyama	POTEKP ACTBL3 FKSG30	ALDSEQEMAMAASSSSVEKsyELPDGQVItIGNERFRCPEA
Q9UER7	S176	SIGNOR\|EPSD\|PSP	DAXX BING2 DAP6	SGNNPPTHLsLDPtNAENtAsQsPRtRGsRRQIQRLEQLLA
Q9UER7	S184	SIGNOR\|EPSD\|PSP	DAXX BING2 DAP6	LsLDPtNAENtAsQsPRtRGsRRQIQRLEQLLALYVAEIRR
Q9Y3F4	S179	EPSD\|PSP	STRAP MAWD UNRIP	LsADDKtVRLWDHATMtEVKsLNFNMSVSSMEYIPEGEILV
Q9Y3F4	T175	EPSD\|PSP	STRAP MAWD UNRIP	DKQILsADDKtVRLWDHATMtEVKsLNFNMSVSSMEYIPEG